动物造模,药效评价,卵巢癌 z-bio 2024-08-01 386

　　[Modeling mechanism] Chemical carcinogens are directly applied to tissues through local embedding and other methods. These carcinogens act on the surface epithelium of the ovary, causing cell transformation. At the same time, they may also cause ovarian tumors by damaging follicles and/or oocytes, altering the levels of gonadotropins.

　　After dissolving DMBA in benzene solvent, immerse the cotton thread in the solution and place it in a fume hood until the benzene evaporates. Calculate the drug content of the cotton thread as 0.5mg/cm. 8-week-old F344 female rats were anesthetized by intraperitoneal injection of pentobarbital sodium. Under sterile conditions, a longitudinal incision of about 1.5cm was made in the middle of the lower abdomen and 1cm above the pubic symphysis. After fully exposing the ovaries, a drug containing cotton thread was sewn into the rat ovary using absorbable suture No. 5, and the abdomen was closed. Every week, 0.1ml of gentamicin is injected intraperitoneally into each rat.

　　Other methods: Using talcum powder in the perineum can lead to carcinogenic effects by reaching the surface of the ovaries through the reproductive tract; Directly applying talcum powder to the surface of the ovary can cause local papillary changes and ovarian cysts. The chemical DMBA can induce epithelial tumors on the surface of rat ovaries, and mutations in p53 and K-ras genes were also detected. Exposure of rhesus monkeys and macaques to insecticides such as hexachlorobenzene pollution can induce ovarian failure, significant changes in ovarian size and shape, and alterations in the arrangement of OSE levels.

　　【 Model features 】 Tumor formation began at the 6th week after surgery, and there were metastases in the peritoneum and other areas. Typical clinical manifestations of ovarian cancer in modeling animals include emaciation, frog like abdomen, bloody ascites in the abdominal cavity, anemia (pale eyeballs, pale earlobes), and exhaustion. Stimulating the ovaries of rodents with chemical substances can produce toxicity and carcinogenicity, leading to the withering of follicles and the development of granulocytic tumors and benign mixed tumors.

　　Model evaluation and application: The local action of chemical drugs is a relatively direct method of inducing tumors, but it requires surgical and other procedures on experimental mice, which can easily cause infection and even death. Meanwhile, if the dose is too small, its incubation period can exceed the lifespan of the animal, leading to a decrease in the carcinogenic rate. This animal model is similar to human ovarian cancer in terms of tumor growth patterns, disease progression, complications, etc., making it an ideal animal model for ovarian cancer.