动物造模,药效评价,人卵巢癌裸鼠移植瘤模型 z-bio 2024-08-01 331

　　The nude mouse transplantation model of human ovarian cancer is an ideal carrier for ovarian cancer research, which has important applications in the etiology, pathogenesis, biological characteristics, radioimmunoassay diagnosis, screening of chemotherapy drugs, gene therapy, immunotherapy, and development of anti-tumor drugs of human ovarian cancer.

　　[Modeling mechanism] Due to congenital thymus deficiency and cellular immunodeficiency in nude mice, allogeneic tissue implantation rarely results in rejection reactions. This lays the biological foundation for the stable implantation of human tumor tissue in nude mice. Using human ovarian cancer resection specimens and human ovarian cancer tumor cell lines, inoculated subcutaneously, intraperitoneally, and in the ovaries of nude mice, primary human ovarian cancer nude mouse transplant tumor models, human ovarian cancer nude mouse subcutaneous transplant tumor models, human ovarian cancer nude mouse intraperitoneal transplant tumor models, and human ovarian cancer nude mouse primary transplant tumor models can be established.

　　[Modeling Method] BALB/c-nu/nu nude mice, 4-6 weeks old, female, are commonly used as experimental animals. Collect human ovarian cancer resection specimens, prepare cells, culture them routinely, collect logarithmic growth stage ovarian cancer cells, adjust the cell density to 5.0 × 10000000 cells/ml, and inject 0.2ml/cell intraperitoneally.

　　【 Model Characteristics 】 After 19-40 days of vaccination, nude mice showed significant increase in abdominal circumference, palpable tumors in the abdomen, reduced activity, and weight loss. After euthanizing the nude mice with dislocation, the abdominal cavity was opened to reveal the presence of metastatic solid tumor tissue. Pathological examination was performed on the tumor tissue and ascites, and under the microscope, large and deeply stained cancer cell nuclei were observed, with visible nuclear division and nucleoli. Some areas of the tumor tissue showed coagulative necrosis.

　　The success rate of primary human ovarian cancer tissue transplantation in nude mice is relatively low, ranging from 28% to 40%. Once the primary transplantation is successful, the success rate of transplantation between mice through tissue blocks or cell passaging can reach 100%, and the incubation period is shortened, the tumor growth rate is faster, and the animal experimental period can be shortened. Ascites transplantation tumor reflects the characteristics of ovarian cancer cells growing in the abdominal cavity and forming ascites, which is consistent with the clinical manifestations of human ovarian cancer to a certain extent. It provides a reliable research carrier for drug selection and biological therapy in intraperitoneal chemotherapy. However, there is often a lack of solid tumor expression in the ovary, and the survival time of ascites transplantation tumor models is short. In recent years, it has been mainly used in research such as intraperitoneal chemotherapy and radiation immunotherapy.