动物造模,药效评价,非细菌性前列腺炎 z-bio 2024-08-01 507

　　Chronic non bacterial prostatitis may be caused by mycoplasma, chlamydia, and autoimmune diseases, and its clinical symptoms are very similar and non-specific to those of chronic bacterial prostatitis. The main symptoms include urgency, pain, incomplete urination, and pain, soreness, and swelling in the perineum. Chemical inflammation is caused by local injection of chemical agents into the prostate gland. Alternatively, biological agents can be used to stimulate immune responses in model animals, leading to immune inflammation in prostate tissue.

　　[Method of Modeling]

　　1. A set of routine surgical instruments for rats and C57BL/6 mice, sterile microsamplers, anesthesia supplies, and corresponding chemical products such as carrageenan, Xiaozhiling, formaldehyde, croton oil, glycerol, and 2% agar. Or complete Freund's adjuvant (CFA) or pertussis vaccine.

　　2. Under anesthesia and sterile conditions, male rats underwent a midline incision in the lower abdomen to expose the prostate gland. According to experimental needs, an appropriate amount of pro-inflammatory agent was injected into the prostate gland using a micropipette. Alternatively, mice can be intraperitoneally injected with 0.5ml of 3mg/ml CFA, followed by subcutaneous injection of 1mg of purified prostate antigen protein the next day, and intraperitoneal injection of 0.1ml of pertussis vaccine. Prostatitis can be formed after 8 weeks.

　　3. Sample collection and observation indicators: Observe the condition of prostate lesions in the lateral lobe with naked eyes; Weigh the wet weight of the lateral lobe prostate and calculate the relative weight; Total white blood cell count and phospholipid body density in prostate fluid; Prostate pathological section examination is scored based on the degree of inflammatory cell infiltration and fibroblast proliferation in the prostate stroma, the size of the prostate gland cavity, and the amount of secretion in the gland cavity. The degree of inflammatory cell infiltration and fibroblast proliferation in the prostatic stroma is scored in 4 levels, while the size and secretion of the prostatic gland cavity are scored in 3 levels. The differences in histopathological scores between different treatment groups are compared, and statistical analysis is conducted to determine the drug effect. [Model features] The prostate has significantly increased in weight, and microscopic examination shows varying degrees of damage to the prostate tissue structure. There is uneven tissue proliferation or atrophy, ductal dilation or damage, partial basement membrane destruction, increased or decreased secretion, interstitial edema of the prostate in rats with a large number of inflammatory cells infiltrating lymphocytes, monocytes, and neutrophils, decreased or disappeared secretion in the glandular cavity, and a large number of inflammatory cells and shed glandular epithelial cells.

　　The pathogenesis of this type of animal model is acute chemical inflammation, with rapid and severe pathological reactions, and even widespread necrosis of prostate tissue. These manifestations are not very consistent with clinical manifestations, and there are significant differences in pathological manifestations, lacking pathological specificity. The pathological changes of chronic inflammation were confirmed in the prostate specimens of experimental animals through pathological sectioning and electron microscopy observation, which are similar to the pathological changes of chronic non bacterial prostatitis in humans. At the same time, some glands of experimental mice, such as thymus, submandibular gland, and spleen, were observed, and no pathological changes similar to prostate were found, indicating that this experimental method has good specificity. This model can be used for the study of the pathogenesis, drug screening, and efficacy evaluation of chronic non bacterial prostatitis.

　　In addition to injecting chemical or biological agents to induce non bacterial prostatitis models, other methods include: ① castration and estrogen induced prostatitis in rats; ② Mouse neonatal autoimmune prostatitis model with thymus removal; ③ Genetic aging rat spontaneous prostatitis model; ④ Lactic acid pelvic floor injection method, etc.