动物造模,效评价,牙周炎 z-bio 2024-08-01 439

　　The clinical characteristics of periodontitis mainly include gingival inflammation, periodontal pocket formation, alveolar bone resorption, and tooth loosening and displacement. Among them, how to prevent further alveolar bone resorption and promote new bone formation in periodontal defect sites is the top priority in the treatment of periodontitis. This model simulates alveolar bone defects during periodontitis by mechanical bone removal, and is mainly used for research on the treatment of alveolar bone defects.

　　[Modeling Method] After fasting for 8 hours, experimental dogs were injected with Su Mian Xin II (0.05ml/kg) into the thigh muscles, and fixed on the operating table after anesthesia. Disinfect the oral mucosa with iodine, open the mucoperiosteal flap in the buccal molars of the upper and lower jaws, use a high-speed turbine cylindrical needle to remove the bone and dentin from the enamel bone boundary to the root on the buccal side, with a height of 5mm, a width of 2mm in the mesial and distal directions of the root bifurcation area, and a depth of 3mm in the buccal lingual direction. Use orthodontic ligatures to ligate the neck of the teeth, and reposition and suture the gingival flap. After the dog wakes up, retract it and remove the stitches 7 days later.

　　On the basis of ligation and mechanical bone removal, the modeling time can be greatly shortened. In addition, due to the consistent amount of bone removal, the model standards have strong uniformity, but theoretically, they are not as close to the human body as the naturally occurring periodontal disease model.

　　【 Model Evaluation and Application 】 Periodontitis is a slow and gradual process of onset. If natural disease factors are selected to replicate the periodontitis model based on this characteristic, it will have greater similarity with the human body. However, there are often shortcomings such as long experimental cycles and difficulty in unifying model standards. If the method of manually removing alveolar bone is used, combined with several other pathogenic factors, it can accelerate the replication process of periodontal disease models. Due to the consistent amount of bone removal, the model standards have strong uniformity, but theoretically, it is not as close to the human body as the naturally occurring periodontal disease model. However, the animal model of canine mechanical injury to periodontitis has been widely used in the study of bone defect mechanisms and local treatment of periodontitis due to its unique advantages. Dogs also have two sets of teeth, deciduous and permanent, with wide interdental gaps, making them suitable for artificial periodontal pocket models. They are affordable and easy to control, making them ideal animals for establishing experimental periodontitis models. Especially, the use of Beagle to establish canine periodontitis models has been widely accepted by scholars at home and abroad.