动物造模,药效评价,肺源性心脏病 z-bio 2024-08-05 365

　　[Operation steps] Select adult Japanese large eared rabbits and inject a 1% mass fraction of ferric chloride aqueous solution into the rabbit ear vein. Inject 0.5ml twice a week from week 1 to week 4; From the 5th to the 6th week, twice a week, 0.75ml each time; In the 7th week, 4 times a week, 1ml each time, can complete the mold making.

　　[Result analysis] Ferric chloride can increase blood viscosity, damage vascular intima, cause pulmonary artery fibrosis and sclerosis, cause pulmonary hypertension, pulmonary heart disease, cause hypoxia, carbon dioxide retention and acidosis, and further damage multiple organs such as heart, lung, liver, kidney, etc. The main pathological manifestations include myocardial edema and necrosis; Thickening of pulmonary artery wall and proliferation of collagen fibers; Severe edema and punctate necrosis of liver cells; Glomerular congestion, tubular swelling, and interstitial congestion. The myocardial fibers show a large contraction band and the mitochondria are condensed; Significant thickening of alveolar walls, fibrous connective tissue proliferation, partial occlusion of capillary lumens, and fibrous connective tissue proliferation in the smooth muscle interstitium of pulmonary arterioles; Hepatic cell edema, partial mitochondrial condensation, fibrosis between liver lobules, and infiltration of interstitial lymphocytes; Glomerular fibrosis is evident, with membrane bodies observed in some renal lumens.