动物造模,药效评价,口蹄疫 z-bio 2024-08-05 366

　　(1) The replication method involves diluting the foot-and-mouth disease virus ORMF8 with 0.01mol/L PBS (pH 7.4) to an LD50 of 10 ^ -3 to 10 ^ -7/0.2ml. Subsequently, each titer of virus solution is injected intradermally into the hind limbs and toes of guinea pigs, with an injection volume of 0.2ml per animal.

　　(2) The model features that guinea pigs infected with foot-and-mouth disease virus can cause regular and typical onset of disease, which is the key to successfully establishing a guinea pig foot-and-mouth disease virus infection model. Previous studies have shown that guinea pigs have been successfully used as experimental animals for foot-and-mouth disease infection for a long time. Newborn mice are highly sensitive to foot-and-mouth disease virus, but their sensitivity decreases after about 5 days of age. In addition, adult mice and hamsters are also sensitive to foot-and-mouth disease virus, but the onset does not seem to follow the pattern of guinea pigs. Infection of guinea pigs with foot-and-mouth disease virus can cause typical disease characteristics and significant pathological changes; The diseased guinea pig may develop small blisters at the vaccination site, which may then be absorbed and disappear without leaving a ulcerated surface. However, secondary blisters may occur in the limbs, toes, or oral cavity of the guinea pig. The guinea pig may become emaciated and some may even die.

　　(3) In comparative medicine, the original foot-and-mouth disease O-type inactivated vaccines in China were tested for efficacy using guinea pigs (or pigs and cows). The International Organization for Animal Health (OIE) advocates using guinea pigs for vaccine efficacy testing, but also suggests that alternative methods with parallel relationships can be used as routine testing methods. The extensive use of guinea pigs for vaccine testing is costly, time-consuming, and labor-intensive. When the experimental data is sufficient and reliable, using non animal methods for testing is in line with the development trend of miniaturization and non animal testing of organisms. Guinea pigs have been successfully used as model animals for artificial infection of foot-and-mouth disease virus for a long time. They can not only be used for antibody detection, but also for evaluating the immune efficacy of foot-and-mouth disease vaccines through clinical symptoms and pathological observation.