动物造模,药效评价,癫痫 z-bio 2024-08-06 365

　　1. Modeling material animals: SD rats, half male and half female, weighing (300 ± 20) g; Drug: Penicillin.

　　2. Modeling method: The model group was intraperitoneally injected with 7 million u/kg penicillin, while the blank group was simultaneously intraperitoneally injected with an equal amount of physiological saline.

　　3. The possible mechanism by which penicillin can cause epileptic seizures is that it can inhibit GABAergic neurons, causing a decrease in inhibitory synaptic activity or an increase in excitatory synaptic activity, resulting in an increase in neuronal excitability. When the excitatory postsynaptic potential of neurons exceeds a certain threshold, paroxysmal depolarization drift can occur, which is closely related to Ca2+. PDS is one of the main features of epilepsy electrophysiology, and the spike waves recorded by EEG are the response of PDS related action potentials.

　　4. Changes after modeling: Before modeling, rats were active, had a normal diet, had agile reactions, and had pure white and smooth fur. After intraperitoneal injection of penicillin, the animals gradually became quiet, followed by dull eyes, increased breathing, nodding, shaking of head muscles, or sudden body twitching. After 20-30 minutes, mild cases may experience single or multiple limb twitches, limb separation, center of gravity descent, tail curling, and uncoordinated crawling; In severe cases, it manifests as strong muscle twitching throughout the body, sudden running, jumping, falling, and continuous convulsions leading to death. The latency period of epileptic seizures in the model group was (50.81 ± 11.43) seconds.