动物造模,药效评价,眼内膜炎 z-bio 2024-08-06 360

　　(1) The replication method involves culturing Staphylococcus aureus ATCC29213 strain and preparing a bacterial suspension of 10000 CFU/ml using physiological saline. Healthy adult New Zealand rabbits were anesthetized by intramuscular injection of a mixture of ketamine (at a dose of 35mg/kg body weight) and lidocaine (at a dose of 20mg/kg body weight). After anesthesia, the animals were fixed and the conjunctival sac was washed with physiological saline; Subsequently, a 1ml syringe was used to aspirate 0.1ml of vitreous from 2mm behind the limbus of the rabbit's temporal quadrant, and 0.1ml of a known concentration of bacterial solution was injected into the eye at the same location. After surgery, a mixture of 0.1% gentamicin and dexamethasone was used for eye drops, four times a day.

　　(2) Model features: 1 hour postoperative conjunctival congestion and edema, 12 hours postoperative corneal edema, anterior chamber exudation, and reduced pupil response to light in model rabbits; Mild corneal opacity and iris congestion within 24 hours; At 3 days, the vitreous body is cloudy, at 7 days, there is posterior adhesion of the iris, at 10 days, the cornea is severely cloudy, the vitreous body is gray white cloudy, at 14 days, the cornea is cloudy, the iris cannot be seen clearly, and the vitreous body is gray white cloudy. Microscopic pathological observation showed that at 1 hour and 12 hours after surgery, the iris and ciliary body of the model animal were congested, inflammatory cells infiltrated the atrial horn, choroidal congestion, and protein like exudation appeared in front of the retina; At 24 hours, there was exudation in both the anterior and subretinal regions of the retina, with a large number of inflammatory cells on the surface of the iris and corneal edge; During 3D, the animal developed panoculitis, with a large amount of exudation at the corneal edge, swelling of the ciliary body, dilation of iris capillaries, significant exudation from the anterior chamber, and a large number of inflammatory cells visible in the vitreous cavity. The retina also shed, and some ganglion cell layers were infiltrated with inflammatory cells. After 7 days, the inflammatory response decreased and a large amount of inflammatory cell infiltration was observed in the retina.

　　(3) Comparative medicine endometritis is a serious disease that threatens human vision in clinical practice, often caused by bacterial infections. Its main pathogens include Bacillus cereus and Staphylococcus aureus, and the common causes are intraocular perforation or intraocular surgery. The model of endophthalmitis replicated by this method is similar in the occurrence process and pathological characteristics of inflammation to human endophthalmitis caused by bacterial infection in clinical practice, and can be used for research on the pathogenesis and drug screening of endophthalmitis.