动物造模,药效评价,慢性疼痛 z-bio 2024-08-06 351

　　1. Modeling material animals: rabbits, weighing 2-3kg, male or female not limited; Drug: Anesthetic; Instrument: Electrical stimulator.

　　2. The modeling method involves using polytetrafluoroethylene insulated multi stranded silver wire as bipolar electrodes, which are aseptically implanted onto the left visceral nerve of the animal under anesthesia. One week after the surgery, the experiment began by placing the animals in a rabbit box with their heads and limbs freely movable. After the animals adapted to a quiet environment, they began to stimulate the large visceral nerves and measure the pain threshold. The stimulation parameters are short string unidirectional square waves, with a string length of 500ms, a wave width of 1ms, a frequency of 40Hz, and an intensity that increases by about 0.1mA every 5 seconds. When the stimulation intensity increases to a certain value, rabbits exhibit behavioral reactions, mostly manifested as crawling or contracting of the forelimbs, tilting the head or turning the head to one side, sometimes simultaneous forelimb activity and head turning, and a few rabbits may cause systemic activity reactions. The minimum stimulus current intensity that triggers behavioral response is used as the visceral pain threshold, measured every 5 minutes for a total of 3 times, and the mean is used as the baseline threshold for each experiment.

　　3. Modeling principle: Electrical stimulation of visceral nerves induces pain responses in animals.

　　4. After modeling, changes in electrical stimulation can cause animal pain reactions, namely behavioral reactions, mostly manifested as crawling or contracting of the forelimbs, tilting the head or turning the head to one side, sometimes simultaneous forelimb movement and head turning, and a few rabbits may cause systemic activity reactions.