动物造模,药效评价,疼痛模型 z-bio 2024-08-06 341

　　1. Modeling material animals: rabbits, weighing 1.5-2kg; Drug: Anesthetic; Equipment: dental drill, platinum electrode, linear ramp function stimulator.

　　2. The modeling method involves anesthetizing the animal and using a high-speed dental drill with a No.1 drill bit to create a hole near the gingival line of the front incisors, exposing the medullary cavity. A fine platinum electrode is inserted into the medullary cavity, and then the hole is filled with silver amalgam to form a chronic buried electrode. The experiment can be conducted one day after animal surgery. Using a linear ramp function stimulator as the voltage source, direct current with a frequency of 0.33V/s is used to stimulate with a linearly increasing voltage (5V, 10-20V, 30-40V electrical stimulation intensity can also be selected for experiments).

　　3. Modeling principle: Electrical stimulation of the dental pulp causes animal pain response.

　　4. After modeling, electrical stimulation is used to induce tongue licking or chewing reactions (pain reactions) in animals (which may also include pain reactions such as raising the nose, extending the head forward, lifting up, turning sideways, and swinging). The average of three consecutive measurements is taken as the control threshold voltage. If the pain threshold of the control rabbit is greater than 5V, it should be discarded. After the pain threshold is established, the reaction values of the experimental animal after medication should be measured accordingly, with 10V as the limit value, measured every 10 minutes for a total of 1 hour.