动物造模,药效评价,三叉神经痛 z-bio 2024-08-07 441

　　1. Modeling material animals: SD rats, weighing 250-300g, male or female not limited; Drug: 5% formaldehyde.

　　2. Before conducting the modeling experiment, the animal should be kept in a quiet environment for at least 24 hours. Place the rats in a glass observation box (20cm × 20cm × 30cm) with a partition made of organic glass. Place a mirror at the bottom of the box to observe changes in rat behavior. After the animal becomes familiar with the environment for about 30 minutes, it changes from the initial exploratory state to a resting state. Then lure the animal into a cloth pocket so that the mouth and face can be exposed. Subcutaneous inject 50 μ l of 5% formaldehyde solution into the left upper lip and nose side of the animal (the distribution area of the trigeminal nerve endings) using a 1ml syringe. After injection, place the animal back on the observation table and observe the pain behavior response by timing. The time (S) for the animal to scratch the injection site is used as the pain sensitivity behavior observation indicator. The observation is recorded for 45 minutes and divided into 15 sections, with each section lasting 3 minutes as the observation scoring period. The degree of pain in each section is scored by timing (s), and the total duration of each scratch is used as the pain sensitivity intensity indicator.

　　3. Modeling principle: Formaldehyde causes trigeminal neuralgia in animals.

　　4. Changes after modeling: About 20-30 seconds after subcutaneous injection of formaldehyde into the left upper lip of modeling rats, the first phase reaction begins within the first 3 minutes. Rats commonly use the left front and back paws to grasp the injection site, with front scratching being more common, and there is also activity of the right front paw when grasping with the left front paw. The average time for the first phase of grasping the left upper lip injection site with the front and back paws was (50.3 ± 3.5) seconds, which was significantly different from the control group. Then there is a relatively stationary period, averaging 10 minutes. The second phase was between the fifth and third stages, approximately between 12-40 minutes after formaldehyde injection, with an average pain sensitivity intensity of (385.3 ± 23.8) seconds, which was significantly different from the control group.