动物造模,药效评价,心肌缺血 z-bio 2024-08-07 383

　　Method 1

　　1. Modeling material animal: rat; Medication: Pituitrin; Equipment: Biological function experimental system.

　　2. Modeling method: Inject 0.5u/kg or 11u/kg of posterior pituitary hormone intravenously into rats, with a volume of 1 or 1.5ml, and complete the injection within 10 or 15 seconds.

　　3. Modeling principle: Pituitrin causes myocardial ischemia in animals.

　　4. Changes after mold making: The waves are high and the S-T segment is elevated within 5-20 seconds after mold making, and even a unidirectional curve appears. After injection, the T wave decreases, becomes flat, biphasic or inverted within 30 seconds to several minutes, and there is no significant change in the S-T segment.

　　5. Precautions: Animals should be selected for experiments where the J point rises or the ST segment rises or falls by more than 0.1mV, and the T wave rises first and then falls flat or inverted. The J point is the junction of the QRS complex and the ST segment, which is called the junction point (J point). The weight of experimental animals in the same batch should not differ significantly.

　　Method 2

　　1. Modeling material animal: Wistar rats, 18-20 months old, male; Medication: 20% Uratan, Pituitrin; Equipment: Biological function experimental system.

　　2. Modeling method: Anesthetize rats with 20% Uratan intraperitoneal injection (5ml/kg) and fix them supine on a fixed plate. Let them stand for 15-20 minutes (based on stable electrocardiogram), and rapidly inject posterior pituitary hormone from the abdominal cavity at a dose of 20u/kg. Immediately observe the changes in ST Ⅱ and T Ⅱ on the electrocardiogram, and use T wave elevation, S-T segment elevation (≥ 0.1mV), and significant slowing of heart rate as the criteria for successful modeling.

　　3. Modeling principle: Pituitrin causes myocardial ischemia in animals.

　　4. After modeling, observe the changes in electrocardiogram ST Ⅱ and T Ⅱ. In the model group, the T wave is high, the S-T segment is elevated (≥ 0.1mV), and the heart rate is significantly slowed down.

　　5. After modeling, the biochemical changes show that the active reactants of peroxidase are brown in color, and the cytoplasm, plasma membrane, and blood cells of normal myocardial tissue are darker in color, showing strong positive reactions; The myocardial fibers are uniformly colored in light brown color, with weak positive reactions. Experimental observations showed that the cytoplasm, plasma membrane, and blood cells of normal myocardial tissue were deeply stained, and positive reactants were arranged in a linear pattern along the long axis of myocardial cells; The myocardial fibers are uniformly light brown in color, with indistinct transverse stripes. The staining of cytoplasm, plasma membrane, and blood cells in the myocardial tissue of the model group was lighter than that of the normal group, and the positive reactants were sparsely distributed in dots; The myocardial fibers are uniformly light brown in color, with indistinct transverse stripes.

　　60 minutes after intraperitoneal injection of posterior pituitary hormone in the model group, peroxidase activity was significantly reduced compared to the normal group (46.38 ± 3.86 in the normal group and 12.38 ± 1.29 in the model group).