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动物造模,药效评价,急性心力衰竭
z-bio
2024-08-09
458
（1） Cat acute heart failure model
1. Modeling material animal: cat, weighing 2-4 kg; Drug: Pentobarbital.
2. Modeling method: After anesthesia with pentobarbital, open the chest, insert a left ventricular tube, and use LV dp/dt max as the index of cardiac contractility. Administer 3% pentobarbital intravenously at a constant rate.
3. The principle of modeling is that pentobarbital causes acute heart failure in animals.
4. After modeling, the LV dp/dt max gradually decreased. When it dropped to 26.66-53.32 kPa/s and showed no upward trend for 5 minutes, it was considered to form an acute heart failure model.
（2） Guinea pig acute heart failure model
1. Modeling material animal: guinea pig, weighing 300-400g; Drug: Pentobarbital.
2. Modeling method: After anesthesia with pentobarbital, open the chest, insert a left ventricular tube, and use LV dp/dt max as the index of cardiac contractility. Administer 1.5% pentobarbital intravenously at a constant rate.
3. The principle of modeling is that pentobarbital causes acute heart failure in animals.
4. After modeling, the LV dp/dt max gradually decreased. When it dropped to 13.33-26.66kPa/s and showed no upward trend for 5 minutes, it was considered to have formed acute heart failure.
（3） Canine acute heart failure model
1. Modeling material animal: dog; Drug: Pentobarbital.
2. Modeling method: After anesthesia with 30mg/kg pentobarbital, open the chest, insert a left ventricular tube, and use LV dp/dt max as the index of cardiac contractility. Slowly inject 20-30ml of 3% pentobarbital within 2-3 minutes to reduce MAP to 6.65-8.0kPa and LV dp/dt max to 25% -30% of the baseline level. Then drip pentobarbital 4-6 mg/min to maintain a stable state of heart failure (which can last for several hours).
3. The principle of modeling is that pentobarbital causes acute heart failure in animals.
4. After modeling, the LV dp/dt max gradually decreased to 25% to 30% of the baseline level, leading to acute heart failure.