动物造模,药效评价,葡萄膜炎 z-bio 2024-08-09 499

　　1. Model making method: Healthy purebred Wister rats, weighing around 200mg, were treated with endotoxin (ET): the active ingredient of ET, lipopolysaccharides (LPS), extracted from Escherichia coli with a purity of 99%. Before use, the rats were dissolved in physiological saline to form a 2mg/ml solution. The left foot pad of the model group rats was injected with LPS 600 μ g/kg.

　　2. Observation indicators and analysis

　　(1) Measurement of aqueous humor: Inject LPS for 24 hours, extract aqueous humor from 3mm behind the corneal edge of rats, mix and immediately send for testing to measure the number of white blood cells and protein content in the aqueous humor.

　　(2) Eye manifestations: Prior to the experiment, both eyes of rats were examined using slit lamps and ophthalmoscopes. After injection of LPS, the eyes were examined every 6 hours, and the results of the examination 24 hours after the experiment were used as experimental eye signs. The examination includes the conjunctiva, cornea, aqueous humor, iris, crystalline lens, vitreous body, and retina of both eyes.

　　(3) Pathological examination: There are a large number of inflammatory cells such as neutrophils, lymphocytes, and monocytes in the iris tissue, and cellulose like exudate in the anterior chamber.