动物造模,药效评价,心气虚证 z-bio 2024-08-09 404

　　(1) Method of replication: Adult rats were placed in a 30 ℃ environment for 1 hour, and their tails were cut 0.3-0.5cm and immersed in 37 ℃ warm water. Accurate quantification was performed using a graduated cylinder until 5ml of blood was lost, twice a day, for a total of 10 times. After 5 days of experiment, propranolol 5mg was administered daily to each rat for 5 consecutive days. The next day, anesthesia was administered, and the left ventricular diameter (LVIDd, LVIDs) and left ventricular volume (LVEDV, LVESV) at the end of diastole and end of systole were measured using an ultrasound diagnostic instrument. The left ventricular ejection fraction EF was calculated as [(LVEDV-LVESV)/LVDVDV] × 100%, and the left ventricular short axis shortening rate FS was calculated as [(LVIDd - LVIDs)/LVIDs] × 100%. Blood samples were collected to measure the levels of serum estradiol (E2), testosterone (T), and free testosterone (FT).

　　(2) The characteristics of the model are that the left ventricular short axis shortening rate and left ventricular ejection fraction of the model animals are significantly reduced, indicating a significant decrease in left ventricular systolic function in heart qi deficiency animals. At the same time, the serum estradiol content of the model animals is significantly increased, and the testosterone and free testosterone content are significantly reduced.

　　(3) According to the traditional Chinese medicine theory of "blood and qi are interdependent", "qi is the leader of blood", and "blood is the mother of qi", multiple small bloodletting can deplete qi. On this basis, high-dose administration of propranolol can deplete heart qi and locate qi deficiency in the heart. In addition, using tail trimming to perform bloodletting multiple times is not easy to control the amount of bloodletting, and the trauma to rats is significant after 10 times. This method is theoretically feasible, but the actual replicated syndrome is closer to the deficiency of heart qi and blood, and further experimental verification is needed.