动物造模,药效评价,肾炎 z-bio 2024-08-15 366

　　1. Modeling material animal: Male guinea pig, weighing 400-500g, New Zealand rabbit; Drug: Phosphate buffer solution (pH 7.1), complete Freund's adjuvant.

　　2. Modeling method: Under aseptic operation, the kidneys of rabbits were taken out and perfused with isotonic sodium chloride solution. They were stored overnight at -25 ℃ and slowly melted the next day. The renal medulla was removed, and the cortex was crushed. After passing through a 150 mesh metal sieve, it was placed in a cold container and washed with saline first. Then, it was repeatedly washed with phosphate buffer solution (pH 7.1) and centrifuged at 1500 r/min for 5 minutes. As the glomerulus precipitated faster than the renal tubules, the cell residue was confirmed by microscopy. The upper layer fluid containing abundant renal tubules was mixed with complete Freund's adjuvant to make an emulsifier for the renal tubular basement membrane, which contained 90% of renal tubules and a small amount of glomerulus. Each ml contains 15mg (wet weight) of TBM.

　　Every two weeks, 6 points on the back skin of the modeling animals are selected for subcutaneous injection, with 0.1ml of emulsion injected at each point.

　　3. Modeling principle: Induction of animal nephritis model by heterologous immune complexes.

　　4. Changes after modeling: Animals were euthanized after 32 days of modeling, and swelling of the kidneys was observed with small bruises on the surface. Histological observation shows infiltration of monocytes and giant cells in the interstitium, degenerative and fibrotic changes in the tubular epithelium, and mild damage to the glomeruli. IgG eluted from guinea pig kidneys coagulates with the skin TBM in a test tube, but does not coagulate with the cortical glomerular basement membrane.