动物造模,免结核病,药效评价 z-bio 2024-08-19 327

　　In the past, the preparation of rabbit tuberculosis models was mainly intravenous infection, but now aerosol infection is the most commonly used method.

　　1. Intravenous injection infection model Lurie prepared a rabbit infection model using the method of intravenous injection of commercially available rabbits. In this model, the lung bacterial load reached its peak 4 weeks after infection, but all animal lung tuberculosis cultures were negative 4 months after infection. However, 2/3 of animal lung tuberculosis cultures were positive 6 months after infection.

　　2. Aerosol infection model Yukari C. Manabea et al. prepared the rabbit tuberculosis model by aerosol infection. The strain used was Mycobacterium tuberculosis H37Rv, and the infection doses were 3.57 ± 0.05 log cfu and 3.17 ± 0.21 log cfu, respectively. Four weeks after infection, all experimental rabbits tested positive for OT. Five weeks after aerosol infection, the bacterial load in lung tissue reaches its peak, and granulomas can be observed. After 36 weeks, one-third of infected rabbits had at least one caseous granuloma and positive tuberculosis culture, indicating that the infection was a continuous process. Aerosol infection is currently the most commonly used method for preparing animal models of tuberculosis infection.