动物造模,药效评价,脑水肿 z-bio 2024-08-19 393

　　1. Modeling material animal: rabbit; Medications: Anesthetic, typhoid lipopolysaccharide, 2.5% Evans blue saline solution; Equipment: Brain pressure gauge, multi-channel physiology instrument.

　　2. Modeling method: After animal anesthesia, the common carotid artery is surgically separated, and the right ventricle is intubated. A brain pressure gauge is connected to measure ventricular pressure, or an amplifier can be connected to monitor intracranial pressure (ICP) with a multi-channel physiology instrument. Inject 1ml/kg of 2.5% Evans Blue physiological saline into the ear vein, and after the ICP is constant, inject 4.008mg/kg of typhoid lipopolysaccharide into the right common carotid artery.

　　3. The principle of modeling is that typhoid lipopolysaccharide causes brain edema in animals.

　　4. Changes after modeling: Model animals show symptoms of cerebral edema, and generally can survive for more than 6 hours.