动物造模,药效评价,脑水肿 z-bio 2024-08-19 351

　　1. Modeling material animals: Healthy rabbits weighing 1.75-2.15 kg; Medications: Anesthetics, pertussis bacterial fluid.

　　2. After animal anesthesia, the animal is placed in a supine position and a 5cm incision is made at the midline of the neck to expose the right common carotid artery. The internal carotid artery is separated, and the collateral branches that have not entered the skull are ligated. Then, the starting point of the right external carotid artery is clamped with an arterial clip, and a 4.5-inch needle is used to inject 0.6ml/kg pertussis bacterial solution into the right carotid artery. The injection time is controlled at about 40 seconds, and then the arterial clip is released. After removing the needle, the needle hole is lightly pressed for several minutes to stop bleeding.

　　3. The principle of modeling is that pertussis bacterial solution causes infectious brain edema in animals.

　　4. Changes after modeling: The model animals showed symptoms of cerebral edema. The water content of brain tissue was 78.55 ± 0.65% on the right side and 79.62 ± 0.52% on the left side in the control group; After injecting the bacterial solution for 4 hours, the right side (84.39 ± 1.01)% and left side (80.10 ± 0.81)% of the module were created.