动物造模,药效评价,脑水肿 z-bio 2024-08-19 385

　　1. Modeling material animals: Healthy Wistar rats, weighing 70-100g, male or female not limited; Drug: Lipopolysaccharide.

　　2. Modeling method: The model animals were intraperitoneally injected with 100mg/kg lipopolysaccharide (LPS), while the control group was intraperitoneally injected with 1mg/kg physiological saline.

　　3. Modeling principle: Lipopolysaccharides cause brain edema in animals.

　　4. Changes after modeling: Starting from 2 hours after intraperitoneal administration of lipopolysaccharide, the brain tissue water content of the model animals significantly increased, especially at 6 and 12 hours; The EB content in brain tissue significantly increased, reaching its peak at 6 hours and lasting for 24 hours.

　　Changes in capillaries: After 6 hours of modeling, there was slight swelling of the glial protrusions around the capillaries, with some showing vacuolar changes, and more abundant swallowing and drinking vesicles; 12 hours after modeling, endothelial cells underwent degeneration, cytoplasmic vacuolar changes, disappearance of basement membrane, and severe edema of glial cell protrusions around capillaries.

　　Degenerative changes in nerve cells: nucleolus disappearance, sparse chromatin, coarse endoplasmic reticulum degranulation, blurred three-dimensional lines, disordered cristae, swelling and vesicular appearance, Golgi apparatus vesicular expansion, numerous vacuolar structures around the cell body, high-density electronic fragments around the nerve felt, protrusion swelling degeneration and vesicular appearance.

　　Degenerative changes in glial cells: 6 hours after modeling, the cytoplasm swells, the cell body becomes transparent, organelles disperse, and the protrusions around glial cells swell; 12 hours after modeling, there was severe edema and vacuolization around the glial cells.