动物造模,药效评价,脑缺血 z-bio 2024-08-19 426

　　1. Modeling material animal: rat; Medications: Anesthetics, Antibiotics; Equipment: Electric condenser.

　　On the first day of animal anesthesia, the skin was cut open behind the occipital bone to expose the fenestrae on both sides of the first cervical vertebra. An electrocoagulation device with a tip diameter of 0.5mm was inserted into the fenestrae to burn the bilateral vertebral arteries, causing permanent occlusion. On the second day, a midline incision in the neck was made to expose the bilateral common carotid arteries and ligate them to prepare a global cerebral ischemia model. Opening both common carotid arteries can restore blood flow.

　　3. The principle of modeling is to close four blood vessels in animals, leading to global cerebral ischemia.

　　4. Changes after modeling: Consciousness impairment and disappearance of righting reflex were observed in the modeling rats, indicating the establishment of the model.