动物造模,药效评价,脑缺血 z-bio 2024-08-19 352

　　1. Modeling material animals: Male SD rats weighing 280-350g; Medications: Chloral hydrate, antibiotics.

　　2. Modeling method: Anesthetize by intraperitoneal injection of 10% chloral hydrate (300mg/kg), fix it in an upward position on a rat plate, make a 2cm long skin incision along the center of the neck, separate the right common carotid artery and thread two sutures for later use, then separate the external carotid artery and ligate it. Carefully separate the internal carotid artery and its adjacent small branch (pterygopalatine artery) below the external carotid artery, thread a suture under the branch, and ligate it near the bifurcation. The proximal end of the separated common carotid artery is blocked with an arterial clip, and the distal end is gently pulled up with suture. A small incision is made on the common carotid artery, and a nylon suture rod heated to a bead shape (0.28mm) is slowly inserted into the small opening. It is then slowly pushed into the anterior cerebral artery (about 20mm), and pulled back about 2mm to reach the opening of the cerebral artery, which is about 17mm long (calculated from the bifurcation of the internal and external carotid arteries). The nylon suture rod is ligated and fixed with suture. Ligature the proximal end with another suture, remove the arterial clip, suture the muscle and skin, and return to the cage for feeding after the surgery is completed. The sham surgery group of rats only isolated and exposed the common carotid artery, external carotid artery, and internal carotid artery, without ligation.

　　3. The principle of modeling is to ligate blood vessels, which leads to cerebral ischemia.

　　4. Changes after modeling: The neurological function score of the model group rats was 2.3 ± 0.8, the infarct size was (21.6 ± 6.7)%, and the brain water content was (81.1 ± 1.3)%. The water content of brain tissue in the sham surgery group was (78.5 ± 0.7)%.