动物造模,药效评价,白色念珠菌肺炎 z-bio 2024-08-19 712

　　(1) Modeling method: Candida albicans was cultured in a petri dish at 35 ℃ for 24 hours, and medium-sized, milky white moist colonies were observed growing on the petri dish. Smear Gram staining microscopy reveals thick film spores. Through fungal bud tube test, Komagada Candida staining plate culture, sugar fermentation experiment, and lactose assimilation experiment, it was further identified as Candida albicans. Select a single colony and prepare a bacterial solution of 2.0 × 10 ^ 9 cfu/ml. Kunming clean grade mice were subcutaneously injected with 100mg/kg cyclophosphamide on the back of the mice on days 1, 3, 5, 7, and 9 to prepare immunosuppressive mice. The first group is the control group, in which healthy mice are given bacteria through the airway under normal conditions; The second group consists of immunosuppressed mice, which were inoculated with Candida albicans via airway inoculation. Starting from the third day, the bacteria will be administered daily with a dosage of 0.01ml per individual.

　　(2) Result: No significant pathological changes were observed in the lung tissue of healthy mice in the control group. The second group of mice were euthanized on the 9th day after inoculation, and the lung volume increased, the lung cutting edge appeared obtuse, the color was pale, and there were multiple millet like gray white spots on the surface. Under the microscope, it can be seen that the capillaries in the alveolar wall are highly dilated and congested, the alveolar septum is widened, interstitial edema, alveolar cavity dilation, alveolar epithelial hyperplasia and vacuolar degeneration, and the alveolar cavity is filled with light red edema fluid. Black colored hyphae and thick spore clusters can be found in the alveolar cavity.