动物造模,药效评价,肺气虚证 z-bio 2024-08-20 413

　　(1) Adult rats were replicated by injecting 200 μ g/200 μ L of lipopolysaccharide (LPS) into the trachea on the 1st and 14th days, respectively. From the 2nd to the 13th and from the 15th to the 28th, 5% cigarette smoke was smoked in a smoking box with a volume of 72L every morning for 0.5 hours. Observe the general condition, respiratory rate, and body weight of the animal. After modeling is completed, the animal is euthanized and lung tissue is taken for pathological examination.

　　(2) The model features reduced animal activity, hunched back, decreased weight gain, rapid breathing, frequent coughing, tufting of hair, decreased food intake, delayed movement, reduced weight gain, and increased respiratory rate. The animal's lungs are in an expanded state, with increased volume and slightly uneven lung surfaces, with small vesicular protrusions visible; Partial shedding of tracheal mucosal epithelium, proliferation and hypertrophy of goblet cells and glands, and infiltration of inflammatory cells into the tube wall; Increased and elongated folds in the bronchial mucosa; A large number of neutrophils can be seen in the lumen of the small bronchial tube; There is significant infiltration of inflammatory cells in and around the wall of the tube; Distal narrowing of terminal bronchioles, cystic dilation of respiratory bronchioles and alveolar ducts, alveolar enlargement, thinning of alveolar walls, presenting as central lobular emphysema; Partial peripheral lung tissue shows alveolar enlargement, presenting as alveolar emphysema; The cytoplasm of the columnar epithelial cells of rat bronchial cilia swells, and the cilia decrease and become shorter, with some cilia shedding; Type II alveolar epithelial cells show the emptying of lamellar bodies and vacuolar changes in mitochondria; Macrophages and a few red blood cells can be seen in the alveolar cavity.

　　(3) The symptoms of this animal model in comparative medicine are consistent with the manifestations of lung qi deficiency syndrome. A composite factor method is used to create a lung qi deficiency syndrome model that combines disease and syndrome, which has the characteristics of short time, easy replication, and strong operability, and is also in line with the actual clinical situation.