动物造模,药效评价,结肠炎 z-bio 2024-08-20 288

　　1. Modeling material animals: Newborn 1-day-old Wistar rat pups, male or female, weighing 5-10g.

　　2. Modeling method: First, introduce carbon dioxide into the hypoxic chamber, adjust the flow rate, and use the RSS-5100 portable digital oxygen meter to measure the concentration of carbon dioxide in the chamber to 100% [maintained at (99.9 ± 0.2)%]. Then, place 1-day-old newborn mice in the chamber for 5 minutes and remove them. Quickly introduce oxygen into the oxygen chamber and use the oxygen meter to maintain the concentration in the chamber at 100% [maintained at (99.0 ± 0.8)%]. Finally, place the hypoxic newborn mice in the chamber for 5 minutes and remove them.

　　3. Modeling principle: Hypoxia can reduce mucosal blood flow and induce early changes in capillary endothelial cells, ultimately leading to intestinal necrosis.

　　4. Changes after modeling: The TNF - α content in the homogenate supernatant of the model group (39.957 ± 8.283) pg/mg total protein was higher than that of the control group (33.523 ± 6.752) pg/mg total protein. The NO content in the model group (0.82 ± 0.04) μ mol/mg tissue was higher than that in the control group (0.41 ± 0.02) μ mol/mg tissue.

　　The model group showed positive expression of inducible nitric oxide synthase in inflammatory cells and smooth muscle cells of intestinal tissue, as well as in some intestinal epithelial cells. The number of positive particles and staining intensity were significantly higher than those in the control group, and the positive staining area ratio x average luminosity was 3.01 ± 0.71 according to image analysis; The score range for histopathological changes in the model group is 1-4 points, with a median score of 3 points, ranging from superficial epithelial injury with shortened villi to complete mucosal necrosis.