动物造模,药效评价,肺阴虚证 z-bio 2024-08-20 331

　　(1) Method of replication: Adult mice were treated with 0.1 mg/ml of Levofloxacin Injection at a dose of 0.1 ml/(10 g body weight · d), and 15 mg/ml of Thyroxine Tablets at a dose of 0.1 ml/(10 g body weight · d), administered together with Levofloxacin once daily for 7 consecutive days. The mice were then placed in a glass fumigation box filled with sulfur dioxide gas at a concentration of 0.5 g/m3 for 15 minutes, once daily for 8 consecutive days. At the end of the experiment, samples were taken to observe the nasal cavity, nasopharynx, trachea, and lung tissues. Pathological sections were made using conventional methods, staining was performed, and the pathological changes were observed under an optical microscope.

　　(2) Model characteristics: The degree of nasal inflammation in model animals is mostly moderate to severe inflammation; Mild inflammation can be seen in the nasopharynx of some animals, but no abnormalities are observed in the vast majority; Most model animals show mild to moderate inflammatory changes in the trachea, with less severe inflammation; The pathological changes in the lungs are similar to those in the nasal cavity. Mostly moderate to severe inflammation.

　　(3) Comparative medicine pulmonary yin deficiency syndrome refers to the syndrome characterized by insufficient lung yin, loss of clarity, and endogenous deficiency heat. Its main clinical features are dry cough, low phlegm with viscosity or blood in phlegm, and yin deficiency with internal heat. The nasal cavity of tuberculosis patients with lung yin deficiency syndrome showed significant changes in mucociliary clearance function without conscious symptoms. The nasal cavity and lungs of the model animals with lung yin deficiency syndrome showed similar pathological changes, but there was no significant difference between the nasal cavity and the lower respiratory tract, indicating that there were closely related pathological changes in nasal cavity and lung tissue under the condition of lung yin deficiency. The modeling factors of this method are complex, and further research is needed to determine the syndrome.