动物造模,药效评价 z-bio 2024-08-21 426

　　(1) Copy method: Take HI negative young rabbits weighing 1.5-2.5kg. Each rabbit was intraperitoneally injected with 2ml of 1:10 hepatotoxicity (hemorrhagic virulent FII strain), and their body temperature was measured every 6 hours before and after infection. Perform coagulation test and collect blood samples from the middle ear artery every 6 hours after infection to detect any changes in coagulation test results. Prothrombin time (PT), kaolin partial thromboplastin time (KPTT), platelet count (BPC), and plasma protamine coagulation test (3P test) were all measured using conventional hematological methods; The quantification of fibrinogen degradation products (FDP) was determined using the rabbit FDP indirect hemagglutination inhibition method. Microthrombus detection: Tissue sections were taken from organs such as lungs, kidneys, heart, liver, spleen, brain, adrenal glands, and small intestine of rabbits euthanized at 12 and 24 hours after infection to detect the formation of microthrombi. Take tissue slices from the lungs and kidneys to observe the formation of microthrombi.

　　(2) The animal model of rabbit hemorrhagic disease virus (RHDv) induced DIC is completely consistent with the establishment criteria of DIC model, and has the following characteristics: ① Typical clinical symptoms such as subcutaneous bleeding, mental fatigue, and convulsions. ② The coagulation test shows typical changes in DIC and follows a regular dynamic development process. The high coagulation period occurs 6 hours after infection, and the low coagulation period begins at 18 hours Typical microthrombi can be detected in all organs of the body, with the lungs and kidneys being the most common. The peak period for thrombus detection is 12 hours after infection, and bleeding is the main manifestation at 24 hours or in dead rabbits, showing signs of hyperfibrinolysis. The consistent changes in coagulation patterns of the virus-infected rabbits indicate that RHDV induced DIC in rabbits has high repeatability and good stability, making it an ideal animal model for virus induced DIc.

　　(3) There have been many studies on the establishment of DIC animal models in comparative medicine, and inducers are mostly endotoxins and tissue thrombin. Wigtou successfully established a canine viral induced DIC model by inoculating with canine hepatitis virus, but its onset is slow, observation time is long, and the cost is expensive. And this model has a short onset time, low cost, good repeatability and stability, similar to DIC caused by clinical viruses. The experimental period is short, and the virus is simple and easy to obtain.