动物造模,药效评价,诱发应激性胃溃疡 z-bio 2024-08-21 370

　　(1) Method of replication: Adult experimental rats were fasted and deprived of water for 24 hours. They were anesthetized by intraperitoneal injection of pentobarbital sodium at a dose of 30mg/kg body weight. The animals were immersed in a hot water tank at 80-92 ℃ for 10-18 seconds, with the burn area controlled at 30% -50%. After 3-48 hours, they were euthanized by bleeding and immediately dissected. The method for determining and evaluating the sampling site and gastric mucosal injury index is the same as the water immersion restraint method to induce a stress-induced gastric ulcer animal model, while measuring the content of gastric mucosal cortisol (GC).

　　(2) The damage index of the model animals increased at 6 hours after burn injury, reached its peak at 24 hours, and remained at its peak at 48 hours; The content of gastric mucosal cortisol (GC) also significantly increased at 6 hours after burn injury, with peak values at 12 hours, 24 hours, and 48 hours. The gastric mucosal injury index is positively correlated with the cortisol (GC) content in the gastric mucosa.

　　(3) Comparative medical burns are also a stressor. When animals suffer from extensive burns caused by hot water, similar to various stress factors, it can also lead to increased excitability of the sympathetic nervous system and adrenal medulla, causing constriction of gastric blood vessels, decreased mucosal barrier function, and ultimately triggering the occurrence of stress ulcers. Due to the difficulty in controlling the replication method of this model, the complexity of influencing factors, and the fact that the induced lesions are different from those of human peptic ulcers, it has been rarely used recently.