动物造模,药效评价 z-bio 2024-08-22 489

　　(1) Method of replication: Adult healthy dogs were anesthetized by intraperitoneal injection of pentobarbital sodium at a dose of 40mg/kg body weight; After anesthesia, the animal is fixed supine on the surgical plate, and the abdominal surgical area is routinely disinfected. The abdomen is shaved in the center, and the skin is cut about 2cm along the white line of the abdomen. Sterilized fecal suspension (2ml/kg body weight), Escherichia coli solution, and hydrophilic aeromonas solution (1ml/kg body weight each) are injected into the abdominal cavity, and dispersed as evenly as possible. Then, the abdominal wall was sutured layer by layer, and peripheral blood was collected at 0, 2, 4, 6, and 8 hours after infection to measure white blood cell count (WBC) and neutrophil percentage (N%). At the same time, rectal temperature, mean arterial pressure (MAP), cytokines (TNF, IL-6), and blood endotoxin concentration were measured in the model dog. Model animals become lethargic after infection, lose appetite, have bloated abdomens, and occasionally experience diarrhea. After the autopsy of the model animal, it was observed by naked eye that there was severe infection in the abdominal cavity, with a large amount of purulent and fecal cloudy exudate in the cavity, edema of the intestinal serosa, and congestion and stasis.

　　(2) The characteristics of the model are that the animal feces suspension used for modeling in this method not only contains endotoxins produced by the disintegration of bacterial cells after death, but also serves as a culture medium for the growth and reproduction of live bacteria, and can also have a chemical inflammatory effect on the body; And Pseudomonas aeruginosa is a pathogenic bacterium that can cause certain diseases in humans. Infection can lead to cellulitis, sepsis, and surgical site infections in humans. Therefore, this model uses a certain amount of sterilized suspension, live Escherichia coli, and live Pseudomonas aeruginosa injected into the abdominal cavity of dogs. In addition to causing severe intra-abdominal infections, the model animals can also exhibit systemic inflammatory responses.

　　(3) The animal model of abdominal infection replicated by this method in comparative medicine is similar to spontaneous bacterial peritonitis caused by gram-negative bacteria in humans. The modeling method simulates the occurrence of spontaneous bacterial peritonitis in humans, where bacteria directly penetrate the intestinal wall or migrate to the mesenteric lymph nodes and enter the abdominal cavity. The intestinal bacteria in the cavity then enter the portal venous blood flow and directly enter the systemic circulation through collateral circulation; At the same time, the clinical manifestations of the model animals are similar to those of spontaneous bacterial peritonitis in humans. The method of making this model is simple and has good practical value.