动物造模,药效评价,急性肺损伤 z-bio 2024-08-27 396

　　1. Modeling material animals: Healthy rabbits, male or female, weighing 2.0-2.5kg; Medications: Oleic acid, pentobarbital, heparin physiological saline.

　　2. Modeling method: All animals in each group were fasted for 12 hours before the experiment. After anesthesia with pentobarbital (3mg/kg) through the ear vein, they were fixed on the operating table in a supine position, with an incision made in the center of the neck. The left external jugular vein catheter (containing heparin saline) was separated for infusion, and the right carotid artery catheter was separated for blood collection and blood gas analysis. After animal preparation, a model of acute lung injury (ALI) induced by oleic acid was established by injecting 0.06ml/kg of oleic acid into the ear vein. The control group animals were injected with an equal amount of physiological saline.

　　3. The modeling principle uses oleic acid to establish a rabbit acute lung injury model.

　　4. Changes after modeling: The control group showed no cyanosis before and after the experiment, and there were no abnormal changes in respiratory rate; After intravenous injection of oleic acid into the model rabbits, they all showed restlessness, rapid breathing, and cyanosis, but these symptoms did not improve throughout the entire experiment.

　　Changes in arterial blood gas: Compared with the control group, the model group animals showed a significant decrease in PaO2 30 minutes after intravenous injection of oleic acid, and maintained a low level for 120 minutes.

　　Changes in myeloperoxidase (MPO) activity in lung tissue homogenate: The MP0 content in the modeling group (36.7 ± 6.7) nkat/g was significantly higher than that in the control group (21.7 ± 11.7) nkat/g.

　　After 120 minutes of oleic acid induced injury in the modeling group, the lung weight ratio and lung water content were significantly increased compared to the control group.

　　Under the microscope, the alveolar structure of the control group is clear and there is no exudation. After injury to the model animal, focal pulmonary hemorrhage, interstitial and alveolar edema, and infiltration of inflammatory cells mainly composed of neutrophils were observed under the microscope, and some alveoli were not dilated.