动物造模,药效评价,口腔溃疡 z-bio 2024-08-27 443

　　(1) Copy method: SD rats weighing 150-180g. Take a 6cm long glass tube with a diameter of 3mm at the bottom, and insert a small cotton ball so that the bottom of the cotton ball is level with the bottom of the glass tube. Then, drop 90% carbolic acid solution into the glass tube until the cotton ball is just soaked. Then, the lower end of the glass tube was placed flat on the buccal mucosa on the inner side of the left corner of the rat's mouth. After burning for 30 seconds, white lesions with a diameter of about 3mm were observed on the mucosa in this area. After 24 hours, circular or elliptical ulcers with a diameter of 30-35mm were formed in the experimental area of the rat's left cheek, with a depth of about 0.5mm and a gray white pseudomembrane covering the surface. Observe the redness, swelling, and pain of the oral mucosa several days after administration, and measure the oral temperature. Use a vernier caliper to measure the diameter of the ulcer surface and perform statistical analysis.

　　(2) The characteristics of the model are that the method for treating oral mucosal ulcers in rats induced by carbolic acid is simple, and the damage to the oral mucosa is relatively intuitive. However, oral mucosal ulcers belong to the category of traditional Chinese medicine oral ulcers, tongue ulcers, etc., and there is a distinction between deficiency and excess in syndrome differentiation. The excess cases may be caused by intense heart fire or fumigation by stomach fire, or heat accumulation in the heart and spleen, making treatment easier and less likely to recur after recovery. The model only belongs to this type; Those with deficiency may have insufficient kidney yang and elevated deficiency fire. But most of them are due to the deficiency of organs and yin, with strong virtual fire. Whenever they encounter annoyance, anger, or external heat and evil toxins, it leads to the accumulation of yang qi, the transformation of fire and the production of poison. If the internal dampness and heat are not treated properly, it will become a mixture of deficiency and excess, repeatedly occurring and difficult to cure. Modern medicine generally focuses on local antibacterial treatment, supplemented by symptomatic treatments such as analgesia and anti-inflammatory treatment. Therefore, in order to effectively promote the recovery of oral mucosal ulcers caused by carbolic acid in rats, anti-inflammatory, analgesic, astringent, and saprophytic indicators should be combined.

　　(3) Comparative medicine shows that human oral mucosal ulcers are a common disease in dentistry, and severe pain can affect diet. The syndrome differentiation of this disease often belongs to stomach heat syndrome, which is caused by various reasons. Some are secondary infections or bacterial stomatitis caused by oral mucosal injury, while others are a component of systemic diseases, so they are mostly recurrent oral ulcers. Ulcers with a diameter of 4-15mm have a course of 2-5 years. The treatment methods are mostly a combination of traditional Chinese and Western medicine, such as mixing and grinding ice cream, vitamin B, vitamin C, dexamethasone, erythromycin, metronidazole, lidocaine powder, etc. Dip a cotton swab into a small amount of ulcer powder and apply it to the ulcer surface, 3 times a day. And patients are required to eat more fresh vegetables and fruits, eat less fried, fried, and stimulating foods, and rinse their mouths promptly after eating. However, the diet of rats differs significantly from that of humans and cannot fully simulate the complex etiology of oral mucosal ulcers in humans. Differences in etiology can easily lead to false positive or false positive results.