动物造模,药效评价,牙周炎 z-bio 2024-08-27 488

　　(1) The replication method used Keyes 2000 formula feed (56% fructose, 28% skimmed milk powder, 6% flour, 4% yeast, 3% alfalfa powder, 1% animal liver powder, 2% salt) and a small amount of vegetables to feed adult golden hamsters. After 6 weeks, histopathological observation under the microscope showed epithelial cell proliferation below the enamel bone boundary and periodontal pocket formation; White blood cell infiltration can be seen in the inflamed area, with an increase in vascular cavities and a higher number of osteoclasts on the bone surface. The Keyes 2000 formula can indeed form experimental periodontitis (PD). Adult experimental dogs can be fed a soft food formula (dog sausage, potato flour, sugar, and water), and after 3 months of examination, it is confirmed that periodontitis can occur, with a large amount of plaque and tartar accumulation, gingival recession, and an increase in the number of osteoclasts on the surface of the alveolar ridge. Periodontitis can gradually worsen over time.

　　(2) The production mechanism of this model is mainly based on the fact that rough food is beneficial for self-cleaning of the body's tooth surface, while high sugar sticky soft food is not conducive to self-cleaning of teeth and instead promotes the formation of dental plaque and tartar accumulation. Gingival plaque provides bacterial attachment sites, growth conditions, and lower redox potentials for subgingival plaque formation. So feeding animals a high sugar diet can easily cause the formation of gingival plaque, creating conditions for the formation of subgingival plaque and the occurrence of periodontitis. This model has a simple production method and good repeatability.

　　(3) Periodontitis is an infectious and destructive disease in clinical comparative medicine, and is the leading cause of tooth loss in adults. During the onset of periodontitis, plaque plays a major role in tissue destruction. Therefore, the formation of subgingival plaque is a basic condition for the occurrence of periodontitis in the body, because subgingival plaque is mainly composed of gram-negative bacteria, which grow anaerobically, do not decompose sugars, and have the ability to move. They are highly toxic, and their components and metabolites are extremely strong pathogenic factors. The model established using high sugar sticky soft food can cause dental plaque and periodontitis in animals, which is similar to the clinical manifestations of periodontitis patients. It can be used to explore the etiology, clinical process, and drug screening of inflammatory damage in periodontal disease.