【动物造模,药效评价,结石 z-bio 2024-09-02 406

　　(1) Method of replication: Adult dogs, regardless of gender, were anesthetized with 3% pentobarbital sodium intravenously at a dose of 30mg/kg body weight and fixed on the operating table. Routine skin preparation, disinfection, and drape were performed, and a 10cm incision was made in the middle of the xiphoid process, layer by layer into the abdomen. After fully exposing the gallbladder, a needle is inserted from the bottom of the gallbladder to evacuate bile. Then use 5-0 nylon non-invasive needle and thread to make a purse loop suture, insert pre disinfected human cholesterol stones or pigment stones through the incision in the center of the purse, tighten the suture and tie it, clean with physiological saline, and gradually close the abdominal cavity layer by layer. After waking up, the dog was fed for 30 days, and blood was drawn from the femoral vein for serum ALT, TBIL, and BUN measurements. A disinfected 3.5MHz ultrasound probe can also be used to examine the presence of gallstones in the dog's gallbladder. Alternatively, guinea pigs or rabbits can be used, and after anesthesia, human derived cholesterol stones or pigment stones can be implanted into the gallbladder. After 30 days of routine feeding, bile can be extracted for bile components (TCHO, TBIL, total bile acids, mucus, and calcium) and their content determination. Gallstones can be separated and weighed for visual observation, and liver and gallbladder samples can be taken for histological examination.

　　(2) The model features a probe that can detect the size, shape, and dynamic changes of human derived gallstones in the gallbladder of large animals without damage.

　　(3) Comparative medicine, due to the close relationship between the types of gallstones and the composition of food ingested by the body, results in significant differences in the structural components of human gallstones, such as cholesterol stones, pigment stones, and mixed stones. Implanting gallstones with different compositions from humans into animal gallbladder can observe the changes in their growth and disappearance under drug intervention throughout the entire process, which is of great importance for evaluating the efficacy and characteristics of anti gallstones drugs. Among them, the large animal model can implant different types of human derived gallstones into the animal gallbladder according to research purposes and experimental needs, and observe the dynamic process of changes in cholesterol or pigment gallstones in vivo.