动物造模,药效评价,急性肾衰竭 z-bio 2024-09-18 384

　　1. Modeling material animals: Healthy adult rats, male and female not included; Medications: human blood, physiological saline, 50:50 alcohol and dry ice mixture, distilled water, potassium ferrocyanide, Dowex 50-8.

　　2. Modeling method: Take human blood that has been stored in a blood bank for too long, regardless of blood type. Centrifuge and remove plasma and white blood cells. Wash the red blood cells three times with an equal amount of physiological saline, centrifuge and freeze them in a container. Dissolve them in three times the amount of 50:50 alcohol and dry ice mixture, and add half the amount of distilled water to mix. This solution contains 16% to 18% hemoglobin, and the concentration of hemoglobin can be measured on a spectrophotometer. Store in a frozen state without sterilization or disinfection for future use. During the experiment, the frozen hemoglobin is melted and reacted with an equal amount of 1mol/L potassium ferrocyanide to turn it into denatured hemoglobin. It is then compared with the standard ferrous hemoglobin solution for measurement. Only when the potassium ferrocyanide exceeds 5% can the denaturation of hemoglobin be guaranteed. To remove excess potassium, Dowex 50-8 1g can be added to every 5ml of hemoglobin solution. The denatured hemoglobin after removing potassium can be used for injection.

　　The experimental animals were fasted for 24-72 hours before the experiment, and 2g/kg of denatured hemoglobin was injected into the tail vein.

　　3. The principle of modeling is that denatured hemoglobin causes acute renal failure in animals.

　　4. Changes after modeling: The modeling animals showed vomiting, oliguria, and significant bleeding from the mouth, nose, and eyes. Elevated levels of urea nitrogen and blood potassium are similar to symptoms of human renal failure. The mortality rate can reach 93.5%. Histology is visible. Mild edema and degeneration of renal tubules.

　　The period of oliguria is 6-48 hours after modeling, and renal tubular lesions are obvious. The renal tubular cells show scattered turbidity, swelling, necrosis, and atrophy, and sometimes crystals can be seen in the lumen.