动物造模,药效评价,结膜炎 z-bio 2024-09-23 427

　　Bufalin tincture has strong irritant properties and can stimulate the conjunctiva of rabbit eyes, causing a certain degree of damage, thus replicating the acute irritant conjunctivitis model in rabbits.

　　1. Model making method: New Zealand white rabbits weighing 2.2-2.5kg, both male and female. Take healthy New Zealand white rabbits with excluded eye diseases, and inject 50 μ l of bufotalin tincture (10g bufotalin herb/L) into each rabbit eye using a microsampler, once a day for three consecutive times. After the last stimulus, the participants were randomly divided into groups according to the experimental design and treated accordingly for observation.

　　2. Observation indicators and analysis

　　(1) Symptom observation: After animal induced inflammation modeling, inflammation reactions such as conjunctival congestion, edema, and increased secretion appeared. The scoring criteria for eye irritation response are normal blood vessels, no edema, and no secretion, with a score of 0; Blood vessel congestion appears bright red, with slight edema and a small amount of secretion, 1 point; Blood vessels are congested and appear dark red in color, making it difficult to distinguish blood vessels. There is obvious edema and partial eyelid eversion. Secretions make the eyelids and eyelashes moist or sticky, 2 points; Diffuse congestion appears purple in color, with edema until the eyelids are almost closed. Secretions make the entire eyelid moist or sticky, 3 points.

　　(2) Pathological examination of conjunctiva: On the fourth day after administration, the animals were euthanized and one eye of conjunctival tissue was taken from each animal for pathological observation. The model group showed conjunctival dilation and congestion, with a histopathological observation score of (1.5 ± 0.55) points.