动物造模,药效评价,AIDS z-bio 2024-09-25 429

　　(1) The replication method randomly selected newborn BALB/c mice and intraperitoneally inoculated them with mouse leukemia virus LP-BM5MuLV suspension at a dose of 0.15ml/mouse, with a virus titer of 3 × 10000PFU/ml.

　　(2) Model characteristics: BALB/c mice can develop AIDS after intraperitoneal inoculation of LP-BM5MuLV. The model mice show pathological changes similar to human AIDS, such as lymphadenopathy, hyperimmune proteinemia, immunosuppression and shortened survival time. One month after the main inoculation, the immunoglobulin in the blood of the mice increased significantly, and four months later, the mice could have the clinical manifestations similar to human AIDS, such as significantly enlarged submandibular lymph nodes, significantly reduced Th (CD4+) cells (accounting for 30% of the total number of T cells, while the number of Ts (CD8+) cells remained unchanged), and significantly reduced lymphocyte transformation function of mouse spleen cell suspension stimulated by ConA and Lps.

　　(3) At present, comparative medicine has established AIDS animal models such as mice, cats and monkeys in the field of AIDS research, and has been widely used in relevant experimental research. Since different animal models of AIDS have their own characteristics, researchers need to select appropriate models according to the experimental purposes and conditions. This model is the most simple and practical among the established animal models of AIDS, and is usually used for research on gene therapy of AIDS.