新型糖蛋白丝 z-bio 2025-03-19 294

　　New glycoprotein filament TLP-4 identified in lotus pond water samples from Tsinghua University

　　With the support of the National Natural Science Foundation of China's major research program "Cracking the Sugar Code of Life" (approval number: 92478205), the team of Academician Yan Ning and Associate Professor Yan Chuangye from the School of Life Sciences at Tsinghua University have made progress in the study of advanced three-dimensional structures of sugar. The research results, titled "CryoSeek II: Cryo EM analysis of glycofibers from fresh water reveals well structured glycans coating linear tetrapeptide repeats", were published in the Proceedings of the National Academy of Sciences (PNAS) journal on January 7, 2025. The link to the paper is: https://doi.org/10.1073/pnas.2423943122 .

　　The research team proposed the CryoSeek research strategy and discovered a novel protein filament TLP-4 (Tsinghua Lotus Pond tetrapeptide repeats) with a regular sugar structure. TLP-4 consists of linear tetrapeptide repeat fragments, each containing a conserved 3,4-dihydroxyproline (DiHyp), and both 3-OH and 4-OH are highly O-glycosylated; Adjacent to DiHyp, there exists a conserved serine or threonine modified by O-glycosylation; The periphery of TLP-4 protein filaments is enveloped by sugar chains, and the interactions between sugars play a decisive role in the composition of the protein filaments; These research results provide new ideas for the study of advanced sugar structures.