遗传编码荧光探针 z-bio 2025-04-24 146

　　Figure mScarlet3-H: STED super-resolution images of different organelles

　　With the support of the National Natural Science Foundation of China Youth Science Fund Project (Class C) (Approval No. 32201235) and other grants, Fu Zhifei's team and collaborators at Fujian Medical University have made progress in the research of genetic coding fluorescent probes. The related research, titled "A highly stable monomeric red fluorescent protein for advanced microscopy", was published in Nature Methods on April 17, 2025. Paper link: https://www.nature.com/articles/s41592-025-02676-5 .

　　In recent years, the successful development of multiple green and yellow ultra stable fluorescent proteins has greatly promoted the development of cutting-edge biological imaging technologies. Compared to green and yellow fluorescent proteins, red fluorescent protein has natural advantages, especially in terms of phototoxicity, spontaneous fluorescence, light scattering, and tissue penetration. However, the stability of existing red fluorescent proteins has significant shortcomings, greatly limiting the development of cutting-edge biological imaging technologies.

　　The Fu Zhifei team and collaborators have successfully developed the ultra stable monomeric red fluorescent protein mScarlet3-H (also known as mYongHong, meaning eternal red) using methods such as structural biology and protein artificial evolution. This fluorescent protein has strong chemical stability, thermal stability, and photostability, and is currently the preferred fluorescent probe for optoelectronic correlation imaging technology, rapid transparency imaging technology, and long-term live cell super-resolution imaging technology. The emergence of mScarlet3-H has successfully filled the long-standing gap in the lack of ultra stable monomeric red fluorescent protein.

　　This study demonstrates important achievements in the field of genetically encoded fluorescent probes, providing important labeling tools for cell biology, neurobiology, and other research. It also lays an important research foundation for the development of related functional fluorescent probes.