动物造模,肾性水肿病症大鼠模型 中国实验动物信息网 2022-11-22 1146

　　Objective: To investigate the feasibility of reproducing a disease combined model of renal edema in rats.

　　Methods: The edema model of kidney yin deficiency was established by intragastric administration of thyroxine tablets and tail vein injection of adriamycin, and the edema model of kidney yang deficiency was established by intramuscular injection of hydrocortisone and tail vein injection of adriamycin. The success of the model was judged by observing the changes of physical signs, 24h urine protein content, triiodothyronine (T3), thyroid (T4), cyclic adenosine monophosphate (cAMP), cyclic guanosine monophosphate (cGMP), estradiol (E2) and testosterone (T) in the model rats.

　　Results: The animals in the yin deficiency edema group showed hyperactivity, depilation, dry stool, weight loss and body temperature rise. Compared with the blank group, the contents of T3, T4, cAMP, E2, 24h urine protein increased significantly, the contents of cGMP and T decreased, and the ratio of cAMP/cGMP increased significantly, with significant differences in all indicators (P<0.05). In the Yang deficiency edema group, the animals showed signs of decreased activity, loose stool, decreased body weight and body temperature. Compared with the blank group, the contents of T3, T4, cAMP and E2 were significantly reduced, the contents of cGMP, T, 24h urine protein were significantly increased, and the cAMP/cGMP ratio was reduced, and all indicators were significantly different (P<0.05).

　　Conclusion: The animal disease combined model induced by thyroxine tablets combined with doxorubicin, hydrocortisone injection combined with doxorubicin, respectively, is in line with the TCM syndrome type diagnosis of kidney yin deficiency and yang deficiency edema, and the modeling method is feasible.