动物造模,类风湿关节炎 中国实验动物信息网 2023-06-13 784

　　Objective: To explore the effect of bone marrow mesenchymal stem cells on rheumatoid arthritis and its secondary bone loss using a spontaneous rheumatoid arthritis mouse model.

　　Method 3-week-old Tg ⁃ huTNF α The mice were divided into three groups: (1) model group, with 0.1% saline injected into the abdominal cavity once a week; (2) HuTNF α Antibody intervention group, intraperitoneal injection of 10mg/kg huTNF once a week α Antibodies; (3) BMSCs intervention group (BMSCs), inject 5 times per week into both knee joint cavities × BMSCs of 106Cells. During the intervention period, the animals were weighed once a week and their ankle joints were clinically scored. After 10 weeks of intervention, each group of animals was euthanized for sampling. Analysis of peripheral blood T cells, B cells, and CD4+/CD8+T cell ratios using flow cytometry, and detection of huTNF in serum using liquid chip method α、 IL ⁃ 4 and IL ⁃ 1 β The pathological changes of the left knee joint and ankle joint were evaluated after HE staining, and the bone structure changes of the right femur were analyzed by bone morphometry Metrology.

　　Compared with the model group, BMSCs and Anti ⁃ huTNF α Intervention can alleviate Tg ⁃ huTNF α Weight loss in mice, inhibition of joint swelling and deformation, and downregulation of peripheral blood huTNF α Content, reduce Tg ⁃ huTNF α The joint pathology score of mice reduces the area of joint synovial and cartilage damage. In addition, Metrology analysis of bone morphology found that BMSCs intervention could increase the number of trabeculae and the percentage of trabeculae area, and improve Tg ⁃ huTNF α Mouse femoral bone structure.

　　Conclusion: Intraarticular injection of BMSCs can significantly alleviate Tg ⁃ huTNF α Symptoms of rheumatoid arthritis in mice, improvement of femoral bone structure, and relief of bone loss secondary to RA