动物造模,泻下加劳倦过度脾气虚证 z-bio 2023-09-12 916

　　(1) Reproduction method: Adult rats were raised in a single cage and given a sufficient amount of medicated feed (rhubarb, mirabilite, and standard feed materials were evenly mixed in a ratio of 0.85:0.15:9.0 to make medicated feed, with 0.1g of raw medicine per gram of feed). They were fed freely and oscillated on a shaker from 8 a.m. to 12 a.m. every day for 4 hours (with 20 minutes of oscillation and 5 minutes of stoppage). A total of 3 weeks (21 days) were established to observe the animal's weight, food intake, and the appearance of spleen deficiency.

　　(2) Model characteristics: After 5 days of modeling, the animals showed signs of fatigue, arched back, little movement, squinting (unwilling to open their eyes, upper eyelids covering more than half of the eyeball), pale ear color, loose and messy fur, matte fur, and emaciation. The incidence of arched back was 100%, with pale ear color, messy fur, matte fur, and emaciated fur. Starting from the 3rd day, diarrhea appeared, with mild to moderate symptoms in the first week of modeling, and moderate to severe symptoms after the 2nd week. On the 4th day after discontinuing the feeding of medicated feed, loose stools basically disappeared. During this period, the rate of animal tail pulling and defecation increased, and on the 21st day, the rate of tail pulling and defecation significantly increased. Weight loss occurred in the first week of modeling, and remained stable from the second to third weeks; Food intake decreases in the first week and increases from the second week. Along with the increase in food intake, diarrhea is also more severe, with most reaching severe levels.

　　(3) The comparative medicine model adopts the idea of creating a model of spleen damage caused by bitter cold diarrhea, but reduces the dosage of diarrhea drugs, prolongs the modeling time, avoids acute poisoning caused by a large amount of diarrhea drugs in animals, and uses fatigue factors to promote the formation of spleen deficiency syndrome or exacerbate its damage degree. Using both diarrhea and fatigue factors to create a model that is closer to the actual situation of the formation of spleen qi deficiency syndrome in clinical practice. During the model replication process, it is necessary to further observe the appearance of the experimental animals, and we found signs of injury during the experiment.