动物造模,饥饱失常加劳倦过度脾气虚证 z-bio 2023-09-12 784

　　(1) Reproduction method: Adult rats were raised in a single cage, and the experimental rat cage was fixed to the oscillator using a tray. The oscillator was controlled by a relay to stop for 10 minutes every 20 minutes of vibration, with an average of 10.7 hours of oscillation and 8 days of resonance oscillation per day, or an average of 6.5 hours of oscillation and 28 days of resonance oscillation per day, or an average of 10.2 hours of oscillation and 33 days of resonance oscillation per day, causing fatigue in the animals. Animals are fed with regular mixed feed every other day while oscillating, ensuring sufficient intake. They can freely drink water at any time except for the oscillation time. Observe the general condition of animals, gastrointestinal emptying time, positive rate of defecation, floating rate, swimming time, and changes in nocturnal activity. Conduct electron microscopy of skeletal muscle mitochondria, scanning electron microscopy of gastrointestinal mucosa, ANAE observation of T lymphocytes, cytochemical observation of skeletal muscle glycogen, succinate dehydrogenase and lactate dehydrogenase, and determination of serum leucine peptidase.

　　(2) The characteristics of the model include small squinted eyes, messy hair, matte hair, delayed hair change, arched back, lazy movement, abnormal feces (small or loose), pale ears, gray tail, thin tail, weight loss, increased floating rate, and shortened swimming time. The amplitude and frequency of movements such as standing and walking, scratching, grooming, eyelid washing, and licking the hind legs at night decrease; The emptying time of the digestive tract is shortened, and the positive rate of lifting the tail and defecation is increased; The amplitude of peristaltic waves in the isolated ileum significantly decreased and the frequency increased; The activity of animal serum amylase and serum leucine aminopeptidase increased; The percentage of T lymphocytes decreased. The content of glycogen and succinate dehydrogenase in skeletal muscle decreased, while the activity of lactate dehydrogenase increased; Pathological examination showed a decrease in glycogen content in parenchymal cells of parenchymal organs; The ATP content of red blood cell membrane decreased; Enzymes that undergo conformational changes in ConA receptors on the red blood cell membrane and affect glycolysis. As a result, energy synthesis is insufficient. The weight of organs such as the heart, liver, spleen, stomach, adrenal gland, and thymus decreased, and the thymic cortex became thinner on pathological examination. There is a significant decrease in thymocytes, a decrease in the number and volume of splenic corpuscles in the spleen, and a decrease in the number of lymphoid follicles and marginal zone cells around the central venous sheath; Under electron microscopy, it can be observed that skeletal muscle mitochondria swell, mitochondrial cristae disappear, and the number of mitochondria with normal structure significantly decreases, while those with normal structure significantly increase.

　　(3) Comparative medical fatigue and hunger are important pathogenic factors for spleen deficiency syndrome. In this modeling measure, excessive hunger is due to the inability to receive food. Oversatiety is reflected in the fact that the model animal's overnight food intake is equivalent to 140% of the normal animal's daily food intake. Prolonged exposure to this can lead to metabolic disorders and spleen deficiency in animals. Using hunger, saturation, and fatigue simultaneously can have a mutually reinforcing effect.