动物造模,X射线照射脾气虚证 z-bio 2023-09-12 748

　　(1) Reproduction method: Adult rats were raised in a single cage and subjected to external abdominal irradiation at a dose of 250 rad/min. The first dose was followed by a second dose of enhancement 7 days after the first dose. The changes in food intake, body weight, bowel movements, frequency of tail pulling, resistance to hanging tail pulling, and general condition of the rats were observed. On the 33rd day, blood was collected for serum gastrin, serum D-xylose concentration, serum alkaline phosphatase and isoenzymes, myoglobin, and platelet index, Changes in serum fibrinogen and other factors. The animals were euthanized and the gastric antrum, jejunum, and ileum were taken for light microscopy examination.

　　(2) Model characteristics: Starting from the 11th day, animals experience diarrhea, perianal filth, erect hair, withered hair, decreased appetite, weight loss, fatigue, prone to lying, arched back, increased tail pulling defecation rate, and decreased airborne resistance. The levels of serum gastrin and xylose significantly decreased, while the levels of serum myoglobin and serine threonine kinase (CPK) increased, while the levels of platelets and fibrinogen decreased. Animal dissection showed edema or erosion of the gastrointestinal mucosa, punctate bleeding, and partially visible ulcers of varying sizes. The lesion was mainly found in the gastric antrum, duodenum, jejunum, and ileum. Histological observation of the gastrointestinal mucosa shows an increase in neutral and acidic mucus, mucosal atrophy, edema of the lamina propria, infiltration of inflammatory cells, degeneration and shedding of small intestinal villi, atrophy of intestinal epithelial cells, and interstitial edema of villi.

　　(3) Comparative medical radiation therapy has a relatively good therapeutic effect when applied to the treatment of tumors, but its adverse reactions are severe, and the clinical manifestations are mainly spleen qi deficiency in traditional Chinese medicine. Therefore, X-ray irradiation of the animal abdomen leads to chronic spleen qi deficiency in the animal model, and all indicators are similar to the clinical spleen qi deficiency syndrome. This method has a good idea, and the replicated model is close to clinical practice. However, the most suitable irradiation dose needs to be constantly explored, and experimental conditions are difficult to carry out in some units, lacking universality.