动物造模,莱姆病 z-bio 2023-10-11 981

　　In the investigation and study of the natural host of Lyme disease, it was found that birds play an important role in the long-distance spread of the pathogen. Therefore, chickens were used as models to conduct experimental transmission studies on the vector tick species and animal hosts.

　　1. Modeling method: Pheasants belong to the genus Pheasants, and Lyme disease infection is ruled out. They are raised by professionals at a temperature of 20 ℃. There are two methods for modeling: a group of 4 pheasants, 6 weeks old, subcutaneously inoculated with 1 × 1000 000 Borrelia burgdorferi. The other group selected 5 adults aged 6-8 months, cut off feathers from the throat, back of head, and under the wings, fully exposed the skin beneath the feathers, disinfected the skin with 70% alcohol, and placed them in an experimental room with 80 ticks infected with Lyme disease, so that pathogens can infect pheasants through the bite sensation of infected ticks. When ticks carrying infectious agents bite the host, the swelling of the model animal's body due to ingestion of blood proves that the animal has been bitten. After infection, pheasants showed no obvious symptoms except for a slight increase in body temperature.

　　2. PCR method is used to detect Borrelia burgdorferi DNA in the skin and other tissues of animal models infected with pathogens.

　　3. Model characteristics: The cost of modeling pheasants is high, and there are no obvious symptoms of infection after infection, only showing a slight increase in body temperature. The success rate of using infected ticks to bite pheasants as a model is higher than that of subcutaneous inoculation of pathogens, which confirms that wild birds and animals are prone to being bitten by infected ticks as carriers and carriers of pathogens.

　　At present, various animals have been used to create animal models of Borrelia burgdorferi infection. The commonly used animals include rats, mice, hamsters, and rabbits, which can cause chronic inflammation and symptoms of infection after infection. Intradermal inoculation of Borrelia burgdorferi is a commonly used method for creating rodent models. Spirochetes can be injected intradermally using a syringe (injecting laboratory cultured spirochetes), or through the experimental method of infectious ticks biting the host (using ticks that infect Lyme disease wild animals and infect pathogens through biting). After comparing the two infection methods through experiments, it was found that there is no essential difference and both can successfully create infection models. Most experiments use male animals for modeling, and when female animals are used for modeling, it is found that there is no gender difference in the model manufacturing process, which is consistent with the fact that Lyme disease has no gender difference in human infected hosts. Inbred experimental animals are susceptible to pathogens, and they are more prone to organ lesions such as heart, skin, joints, etc. after being infected with Borrelia burgdorferi, with positive tissue anti spirochete antibodies.