动物造模,兔脑水肿模型 z-bio 2023-10-12 1273

　　1. Animal modeling materials: Healthy rabbits, weighing 1.75-2.15kg; Medications: Anesthetics, pertussis bacterial solution.

　　2. Method of modeling: After anesthesia, the animal is placed in a supine position and a 5cm long incision is made at the midline of the neck. The right common carotid artery is exposed, the internal carotid artery is separated, and the collateral that does not enter the intracranial area is ligated. Then, the right external carotid artery is clamped with an arterial clamp, and 0.6ml/kg pertussis bacterial solution is injected from the right carotid artery using a 4.5 gauge needle. The injection time is controlled at around 40s. Then, the arterial clamp is released, and the needle hole is gently pressed for several minutes to stop bleeding after the needle is removed.

　　3. Modeling principle: Pertussis bacterial solution causes infectious brain edema in animals.

　　4. Changes after modeling: Model animals exhibit symptoms of brain edema. The water content of brain tissue was 78.55 ± 0.65% on the right side and 79.62 ± 0.52% on the left side in the control group; After 4 hours of injection of bacterial solution, the right side (84.39 ± 1.01)% and the left side (80.10 ± 0.81)% were observed in the modeling group.