动物造模,脑水肿模型 z-bio 2023-10-12 853

　　1. Model making material Animal: Rabbit; Medications: Anesthetics, typhoid lipopolysaccharide, 2.5% Evans blue physiological saline; Instruments: Brain pressure gauge, multi-channel physiological instrument.

　　2. Modeling method: After animal anesthesia, the common carotid artery is surgically separated and the right ventricle is intubated. A brain pressure gauge is connected to measure ventricular pressure, and an amplifier can also be connected to monitor intracranial pressure (ICP) using a multi-channel physiological instrument. Inject 2.5% Evans blue physiological saline 1ml/kg from the ear vein, and after ICP remains constant, inject typhoid lipopolysaccharide 4.008mg/kg from the right common carotid artery.

　　3. Modeling principle: Typhoid lipopolysaccharides cause brain edema in animals.

　　4. Changes after modeling: Model animals exhibit symptoms of brain edema, and generally can survive for more than 6 hours.