动物造模,埃博拉病毒 z-bio 2024-02-06 923

　　Many non-human primates have been used in the study of Ebola animal models, including African green monkeys, baboons, rhesus monkeys, and crab eating monkeys. Rhesus monkeys and crab eating monkeys are currently the most widely used, both highly sensitive to EBOV and can cause clinical symptoms very similar to those of humans. Crab eating monkeys are the most sensitive to ZEBOV, with the fastest onset of clinical symptoms, usually 3-4 days. The duration of fever before death is short, ranging from 2-3 days. The clinical symptoms of rhesus monkeys appear slightly slower, but closer to those of humans. Viremia can be detected 2 days after the virus attack and reaches its peak 2 to 3 days later. In addition, baboons have a certain degree of tolerance to all subtypes of EBOV, while African green monkeys are tolerant to REBOV. It is worth mentioning that, in addition to the differences in the types of animal models used, the strains, doses, and vaccination methods used in the attack all have an undeniable impact on the clinical symptoms, duration, and severity of the disease that the model ultimately produces.

　　After being poisoned by ZEBOV, the NHP animal model will experience a sudden decrease in body temperature before death, and the degree of weight loss will also be higher than 10%. It is speculated that dehydration, rather than fat breakdown, may be the cause, and some animals may also experience diarrhea and intermittent black manure. At 4 days after the attack, NHPs usually develop maculopapules on their skin and persist until death. In the early stages of the disease, there may be peripheral lymph node enlargement, while in the middle and late stages, liver enlargement with circular cystic edges can be seen. Viremia can usually be detected 2 days after attack and reaches its peak 2-3 days later. At the same time, all infected NHPs showed significant symptoms of thrombocytopenia, with prolonged clotting time starting from 6 days after the attack and blood loss of agglutination ability by 10-12 days. In addition, the levels of sodium, potassium, and calcium ions in plasma showed a downward trend, while the levels of urea and creatinine increased. Starting from the 5th day, the levels of transaminases (AST, ALT) continued to increase until the animal died.

　　There are few reports on using SEBOV to infect NHPs for animal model research. The clinical symptoms of rhesus monkeys and crab eating monkeys infected with SEBOV appear a few days later than those of ZEBOV, and the survival rate is slightly higher.