动物造模,心肌病模型 z-bio 2023-11-07 1008

　　1. Experimental animal: Wistar rats, two weeks old, regardless of gender.

　　2. Experimental method: Wistar rats, adding 700 furazolidone to drinking water × 10 to the 6th power (adding 700mg of furazolidone per kilogram of water), observed for 12 weeks.

　　3. Experimental observation content measurement method: Ultrasound monitoring of cardiac function. M2 type curves were measured at the left ventricular long axis section and left ventricular papillary muscle level. Left ventricular end diastolic diameter (LVDD, mm), left ventricular end systolic diameter (LVSD, mm), left ventricular end diastolic volume (LVDV), and left ventricular end systolic volume (LVSV) were continuously measured for three cardiac cycles. The average values were taken and converted into left ventricular ejection fraction (LVEF) and left ventricular shortening fraction (LVFS) using the Simpson method, Calculation formula: LVEF=[(LVDV LVSV)/LVDV] × 100%, LVFS=[(LVDD LVSD)/LVDD] × 100%, as a cardiac function parameter indicator.

　　4. Reference results: After 12 weeks of modeling, the mortality rate of rats was 10%, and the success rate of modeling was 66.6%. In the DCM group, myocardial cells in each subgroup of rats proliferate and become hypertrophic, with focal lysis of myocardial cells, varying degrees of granular and vacuolar degeneration, nuclear enlargement, partial division, deformity, widened cell gaps, and normal cell gaps. Research has found that feeding furazolidone for 8 and 12 weeks does not increase the incidence of furazolidone dilated cardiomyopathy. It is speculated that the cardiotoxic effect of furazolidone is age-related, so furazolidone should be given as soon as possible, preferably one week after birth in rats.