动物造模,肾阳虚证动物模型 z-bio 2023-11-20 878

　　(1) Reproduction method: Adult rabbits were randomly inserted into a gastric tube in the morning and administered with a dose of 6mg/kg body weight of aminodexamethasone (distilled water mixed with 10mg/ml suspension) for 15 consecutive days. Daily detailed observation and recording of changes in physical signs, behavior, food intake, water intake, body weight, body temperature, etc., detection of estrous cycle, regular vaginal smear in the afternoon, and cytological examination. On the 16th day, blood was collected from the ear vein for plasma measurement β- Endorphin（ β- EP), euthanize the animals, weigh the uterus and ovaries, and perform histological examination.

　　(2) Model characteristics: No large or mature follicles were observed in the modeling animals, and there was an increase in interstitial fibrous tissue. The endometrium is thin, the glands are sparse, narrow, and straight, and the interstitial cells are dense. The highest value of keratinized epithelial cells and the duration of estrus in modeling animals showed a downward trend, indicating a decrease in estrogen levels. Model animal plasma β- The content of EP significantly decreased, resulting in weight loss and decreased body temperature.

　　(3) Comparative medical amino hypnotherapy can hinder the synthesis of steroid hormones and also affect the degradation and metabolism of corticosteroids, resulting in adrenal cortex dysfunction and decreased function of the gonadal axis.