动物造模,脑瘫 zi-bio 2023-11-23 894

　　1. Experimental cerebral palsy animal model in neonatal rats after asphyxia:

　　Under conscious local anesthesia, newborn rats were subjected to ligation of the right common carotid artery. The animals were then placed in a constant temperature sealed glass container, filled with a mixture of 8% oxygen and 92% nitrogen gas. After 2 hours, they were taken out and observed for signs. The young rats were then divided into groups and decapitated for brain extraction.

　　2. Rat model of central spastic paralysis:

　　Randomly divide the rats with spastic cerebral palsy of the upper limbs into two groups according to the surgical sequence, with 8 rats in each group. Experimental group: C6 nerve roots were cut off.

　　3. Improvement of rat spastic cerebral palsy model:

　　Wister rats, male and female, 200-250g, intraperitoneal anesthesia with pentobarbital 30mg/kg. Make a longitudinal incision through the sagittal suture of the skull top to expose the anterior fontanel. Using an improved de groov positioning system, select 6.6mm behind the anterior fontanel and 1mm to the left of the sagittal suture. Drill a small electric drill with a diameter of about 2mm on the skull plate. Insert the anode needle electrode with a diameter of about 0.5mm vertically into 9.7mm. Connect the cathode to the tail of the mouse and apply direct current stimulation to destroy the vertebral tract. After stimulation, remove the electrode, close the incision, and immediately execute the rat and take the specimen.